Compulsory eligible subject „Current Methods in Cell Biology
(code B81289)

Summer semester 2015/2016

Laboratories of the Institute of Cellular Biology and Pathology

Annotation

This is a one-week practical course in cell biology covering 40 hrs (Monday-Friday, from 8:30 to 16:30) designated first of all for foreign students of the 2nd - 6th year of the Master study programme in the field General medicine.

Place: laboratories of the Institute of Cellular Biology and Pathology, Purkynje Institute, Albertov 4, Prague 2.

First day, the programme begins at 8:15 in the seminar room of the Institute of Cellular Biology and Pathology (Purkynje Institute, Albertov 4, basement).

Teaching will take place in groups in the form of a practical work, in English language. During practical sessions the groups of students will rotate through a defined subset of labs within the institute.

The aim of the course is the familiarization of students with the basic methods in modern cell biology: bacterial and cell cultivation, mammalian cell transfection, immunocytochemical cell protein detection, DNA and mitotic spread preparation, confocal microscopy, preparation of samples for electron microscopy, modern approach to 3D reconstruction of the cell and cell compartments.

The maximum number of students is 20.

The requirements for credit: 40 hrs attendance and short oral presentation (10 min) of selected topic with obtained results, in groups.

Please note: Having applied for the course through SIS, you cannot cancel your application later than 4 weeks before the beginning of the course.

All questions concerning the course can be addresed to: Zdeňka Fischlová, tel.: +420 224 96(8001) email: zfisch@lf1.cuni.cz

Literature:

Literature:

1. Bruce Alberts et al.: Molecular Biology of the Cell (6^th edition), 2014, Garland Science

2. Bruce Alberts: Essential Cell Biology (3^rd EDITION, 2009), Garland Science

3. John H. Wilson, Tim Hunt (5^th EDITION), 2007, Garland Science

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